There is a sentence in the description: \"One accepted method is to review neuronal cells via light microscopy, which is both accessible and non-invasive\".
\nCould anyone explain which non-invasive method were used for the light microscopy?
There is a sentence in the description: \"One accepted method is to review neuronal cells via light microscopy, which is both accessible and non-invasive\".
\nCould anyone explain which non-invasive method were used for the light microscopy?
\n\nMore specifically, you'll use phase contrast microscopy images to train and test your model
\n
https://en.wikipedia.org/wiki/Phase-contrast_microscopy
", "rawMarkdown": "> More specifically, you'll use phase contrast microscopy images to train and test your model \n\nhttps://en.wikipedia.org/wiki/Phase-contrast_microscopy", "votes": null }, { "id": "1546574", "postDate": "10/16/2021 10:04:15", "content": "Right, the question is about meaning of \"non-invasive\" in context of light microscopy. It's still not clear.
", "rawMarkdown": "Right, the question is about meaning of \"non-invasive\" in context of light microscopy. It's still not clear.", "votes": null }, { "id": "1546667", "postDate": "10/16/2021 12:10:49", "content": "perhaps it’s refers that with light microscope you don’t need to add special dyes to the cells, or express special proteins inside the cell that will help visualise cells :)
", "rawMarkdown": "perhaps it’s refers that with light microscope you don’t need to add special dyes to the cells, or express special proteins inside the cell that will help visualise cells :)", "votes": null }, { "id": "1548764", "postDate": "10/18/2021 14:03:00", "content": "DrHB is correct -- typical image-based analysis of cells requires fluorescence-based microscopy, which requires special fluorescent dyes and intense fluorescent light exposure to cells https://en.wikipedia.org/wiki/Fluorescence_microscope. Those dyes and light can cause cells to change behavior, and can even kill them in extreme cases.
\nLight microscopy does not require additional dyes and the light sources are not harmful. However, the resulting images are more challenging to analyze, hence the competition 😊
", "rawMarkdown": "DrHB is correct -- typical image-based analysis of cells requires fluorescence-based microscopy, which requires special fluorescent dyes and intense fluorescent light exposure to cells [https://en.wikipedia.org/wiki/Fluorescence_microscope](url). Those dyes and light can cause cells to change behavior, and can even kill them in extreme cases.\n\nLight microscopy does not require additional dyes and the light sources are not harmful. However, the resulting images are more challenging to analyze, hence the competition 😊", "votes": null } ], "comments": [ { "id": 1546515, "author_name": "mutantspore", "author_url": "", "post_date": "10/16/2021 08:35:01", "content": "\n\nMore specifically, you'll use phase contrast microscopy images to train and test your model
\n
https://en.wikipedia.org/wiki/Phase-contrast_microscopy
", "votes": null, "replies": [ { "id": 1546574, "author_name": "rednikotin", "author_url": "", "post_date": "10/16/2021 10:04:15", "content": "Right, the question is about meaning of \"non-invasive\" in context of light microscopy. It's still not clear.
", "votes": null, "replies": [] }, { "id": 1546667, "author_name": "drhabib", "author_url": "", "post_date": "10/16/2021 12:10:49", "content": "perhaps it’s refers that with light microscope you don’t need to add special dyes to the cells, or express special proteins inside the cell that will help visualise cells :)
", "votes": null, "replies": [] }, { "id": 1548764, "author_name": "tjackson1", "author_url": "", "post_date": "10/18/2021 14:03:00", "content": "DrHB is correct -- typical image-based analysis of cells requires fluorescence-based microscopy, which requires special fluorescent dyes and intense fluorescent light exposure to cells https://en.wikipedia.org/wiki/Fluorescence_microscope. Those dyes and light can cause cells to change behavior, and can even kill them in extreme cases.
\nLight microscopy does not require additional dyes and the light sources are not harmful. However, the resulting images are more challenging to analyze, hence the competition 😊
", "votes": null, "replies": [] } ] } ], "raw_markdown_by_id": { "1546485": "There is a sentence in the description: \"One accepted method is to review neuronal cells via light microscopy, which is both accessible and non-invasive\".\nCould anyone explain which non-invasive method were used for the light microscopy?", "1546515": "> More specifically, you'll use phase contrast microscopy images to train and test your model \n\nhttps://en.wikipedia.org/wiki/Phase-contrast_microscopy", "1546574": "Right, the question is about meaning of \"non-invasive\" in context of light microscopy. It's still not clear.", "1546667": "perhaps it’s refers that with light microscope you don’t need to add special dyes to the cells, or express special proteins inside the cell that will help visualise cells :)", "1548764": "DrHB is correct -- typical image-based analysis of cells requires fluorescence-based microscopy, which requires special fluorescent dyes and intense fluorescent light exposure to cells [https://en.wikipedia.org/wiki/Fluorescence_microscope](url). Those dyes and light can cause cells to change behavior, and can even kill them in extreme cases.\n\nLight microscopy does not require additional dyes and the light sources are not harmful. However, the resulting images are more challenging to analyze, hence the competition 😊" }, "source": "meta" }