![\"\"](\"https://www.googleapis.com/download/storage/v1/b/kaggle-user-content/o/inbox%2F3406049%2F53f56d8383c8a7373f7da57f5cd2145e%2FTrain_control_two_negative_20190630.png?generation=1561883196729891&alt=media\")
Is the case of two negative control wells on a plate allowed?\nAt the data description page, https://www.kaggle.com/c/recursion-cellular-image-classification/data, I could confirm the following description,
\n\n>+ [train/test]_controls.csv In each experiment, the same 30 siRNAs appear on every plate as positive controls. In addition, there is one well per plate with untreated cells as a negative control.
\n\nBut I also confirmed there are two negative control wells on a plate at train_controls.csv like below.\nI would like to confirm whether the number of negative control wells per plate should be >= 1 or =1.
\n\n
Yes, it happens occasionally that a plate will have more than one negative_control well. This occurs as a result of an operational failure to transfer siRNA (whether treatment or positive_control) into the well during the execution of the experiment. Consequently, not every positive_control siRNA appears on every plate, nor does every treatment siRNA appear in every experiment. We should update the data description to reflect this fact.
Thanks for the good question, @sai11fkaneko! I've updated the data description to note that there is at least one negative control per plate.
", "rawMarkdown": "Thanks for the good question, @sai11fkaneko! I've updated the data description to note that there is *at least* one negative control per plate.", "votes": 1 }, { "id": 566182, "postDate": "2019-07-01T21:52:51.363Z", "content": "Thank you so much!\nNow I understand why the second negative_control well appear on a random position.\nIt makes me feel uneasy.
Is the case of two negative control wells on a plate allowed?\nAt the data description page, https://www.kaggle.com/c/recursion-cellular-image-classification/data, I could confirm the following description,
\n\n>+ [train/test]_controls.csv In each experiment, the same 30 siRNAs appear on every plate as positive controls. In addition, there is one well per plate with untreated cells as a negative control.
\n\nBut I also confirmed there are two negative control wells on a plate at train_controls.csv like below.\nI would like to confirm whether the number of negative control wells per plate should be >= 1 or =1.
\n\n
The 30 siRNAs in control are a different set of siRNAs (not part of 1108 target siRNAs ) ? The 30 siRNAs are like a \"reference\" to be used in order to predict the target siRNA ?
", "rawMarkdown": "The 30 siRNAs in control are a different set of siRNAs (not part of 1108 target siRNAs ) ? The 30 siRNAs are like a \"reference\" to be used in order to predict the target siRNA ? ", "votes": 1, "isDeleted": true }, { "id": 566389, "postDate": "2019-07-02T05:58:34.523Z", "rawMarkdown": "", "isDeleted": true } ], "comments": [ { "id": 565984, "author_name": "Berton", "author_url": "", "post_date": "2019-07-01T16:51:23.503000", "content": "Yes, it happens occasionally that a plate will have more than one negative_control well. This occurs as a result of an operational failure to transfer siRNA (whether treatment or positive_control) into the well during the execution of the experiment. Consequently, not every positive_control siRNA appears on every plate, nor does every treatment siRNA appear in every experiment. We should update the data description to reflect this fact.
Thanks for the good question, @sai11fkaneko! I've updated the data description to note that there is at least one negative control per plate.
", "votes": 1, "replies": [] }, { "id": 566182, "author_name": "Fumihiro Kaneko", "author_url": "", "post_date": "2019-07-01T21:52:51.363000", "content": "Thank you so much!\nNow I understand why the second negative_control well appear on a random position.\nIt makes me feel uneasy.
The 30 siRNAs in control are a different set of siRNAs (not part of 1108 target siRNAs ) ? The 30 siRNAs are like a \"reference\" to be used in order to predict the target siRNA ?
", "votes": 1, "replies": [] }, { "id": 566389, "author_name": "", "author_url": "", "post_date": "2019-07-02T05:58:34.523000", "content": "", "votes": 0, "replies": [] } ], "raw_markdown_by_id": { "565984": "Yes, it happens occasionally that a plate will have more than one `negative_control` well. This occurs as a result of an operational failure to transfer siRNA (whether `treatment` or `positive_control`) into the well during the execution of the experiment. Consequently, not every `positive_control` siRNA appears on every plate, nor does every `treatment` siRNA appear in every experiment. We should update the data description to reflect this fact.", "564964": "Is the case of two negative control wells on a plate allowed?\nAt the data description page, https://www.kaggle.com/c/recursion-cellular-image-classification/data, I could confirm the following description,\n\n>+ **[train/test]_controls.csv** In each experiment, the same 30 siRNAs appear on every plate as positive controls. In addition, there is one well per plate with untreated cells as a negative control. \n\nBut I also confirmed there are two negative control wells on a plate at **train_controls.csv** like below.\nI would like to confirm whether the number of negative control wells per plate should be >= 1 or =1.\n\n\n", "566421": "The 30 siRNAs in control are a different set of siRNAs (not part of 1108 target siRNAs ) ? The 30 siRNAs are like a \"reference\" to be used in order to predict the target siRNA ? ", "566389": "" } }