{
  "id": 108115,
  "title": "Morphological Profiling using an image-based assay",
  "url": "/competitions/recursion-cellular-image-classification/discussion/108115",
  "author_name": "",
  "post_date": "2019-09-09T07:49:12.772198500Z",
  "votes": 4,
  "comment_count": 1,
  "views": 0,
  "content": "<p><strong>Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes</strong></p>\n\n<p>After perturbing, staining, and imaging cells, the open-source software CellProfiler is used to extract 1,000+ morphological features of each cell. </p>\n\n<p>The collection of features is known as a profile: it reflects the phenotypic state of the cells in that sample, and can be compared to other profiles to make inferences.</p>\n\n<p><img src=\"https://www.googleapis.com/download/storage/v1/b/kaggle-user-content/o/inbox%2F1107630%2F57e4fce90858ca100e71d8b806b68ce9%2Fmorphological_profiling.png?generation=1568015048361350&amp;alt=media\" alt=\"\"></p>\n\n<p>reference: <a href=\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223290/\">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223290/</a></p>",
  "messages": [
    {
      "id": "622036",
      "postDate": "09/09/2019 07:49:12",
      "content": "<p><strong>Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes</strong></p>\n\n<p>After perturbing, staining, and imaging cells, the open-source software CellProfiler is used to extract 1,000+ morphological features of each cell. </p>\n\n<p>The collection of features is known as a profile: it reflects the phenotypic state of the cells in that sample, and can be compared to other profiles to make inferences.</p>\n\n<p><img src=\"https://www.googleapis.com/download/storage/v1/b/kaggle-user-content/o/inbox%2F1107630%2F57e4fce90858ca100e71d8b806b68ce9%2Fmorphological_profiling.png?generation=1568015048361350&amp;alt=media\" alt=\"\"></p>\n\n<p>reference: <a href=\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223290/\">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223290/</a></p>",
      "rawMarkdown": "**Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes**\n\nAfter perturbing, staining, and imaging cells, the open-source software CellProfiler is used to extract 1,000+ morphological features of each cell. \n\nThe collection of features is known as a profile: it reflects the phenotypic state of the cells in that sample, and can be compared to other profiles to make inferences.\n\n![](https://www.googleapis.com/download/storage/v1/b/kaggle-user-content/o/inbox%2F1107630%2F57e4fce90858ca100e71d8b806b68ce9%2Fmorphological_profiling.png?generation=1568015048361350&amp;alt=media)\n\nreference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223290/",
      "votes": null
    },
    {
      "id": "622165",
      "postDate": "09/09/2019 10:52:34",
      "content": "<p><strong>Introduction to Fluorescence Microscopy</strong></p>\n\n<p>Fluorescence is a process in which matter absorbs light and re-emits at a different wavelength.  Fluorescence is widely used in biological microscopy.</p>\n\n<p>1: <a href=\"https://www.youtube.com/watch?v=AhzhOzgYoqw&amp;t=906s\">https://www.youtube.com/watch?v=AhzhOzgYoqw&amp;t=906s</a></p>\n\n<p>2: <a href=\"https://www.youtube.com/watch?v=iYoAiqufB7w\">https://www.youtube.com/watch?v=iYoAiqufB7w</a></p>\n\n<p>3: <a href=\"https://www.youtube.com/watch?v=gSGq8gOLXwY\">https://www.youtube.com/watch?v=gSGq8gOLXwY</a></p>\n\n<p>4: <a href=\"https://www.youtube.com/watch?v=yI0mG7kh4GY\">https://www.youtube.com/watch?v=yI0mG7kh4GY</a></p>",
      "rawMarkdown": "**Introduction to Fluorescence Microscopy**\n\nFluorescence is a process in which matter absorbs light and re-emits at a different wavelength.  Fluorescence is widely used in biological microscopy.\n\n1: https://www.youtube.com/watch?v=AhzhOzgYoqw&amp;t=906s\n\n2: https://www.youtube.com/watch?v=iYoAiqufB7w\n\n3: https://www.youtube.com/watch?v=gSGq8gOLXwY\n\n4: https://www.youtube.com/watch?v=yI0mG7kh4GY",
      "votes": null
    }
  ],
  "comments": [
    {
      "id": 622165,
      "author_name": "grapestone5321",
      "author_url": "",
      "post_date": "09/09/2019 10:52:34",
      "content": "<p><strong>Introduction to Fluorescence Microscopy</strong></p>\n\n<p>Fluorescence is a process in which matter absorbs light and re-emits at a different wavelength.  Fluorescence is widely used in biological microscopy.</p>\n\n<p>1: <a href=\"https://www.youtube.com/watch?v=AhzhOzgYoqw&amp;t=906s\">https://www.youtube.com/watch?v=AhzhOzgYoqw&amp;t=906s</a></p>\n\n<p>2: <a href=\"https://www.youtube.com/watch?v=iYoAiqufB7w\">https://www.youtube.com/watch?v=iYoAiqufB7w</a></p>\n\n<p>3: <a href=\"https://www.youtube.com/watch?v=gSGq8gOLXwY\">https://www.youtube.com/watch?v=gSGq8gOLXwY</a></p>\n\n<p>4: <a href=\"https://www.youtube.com/watch?v=yI0mG7kh4GY\">https://www.youtube.com/watch?v=yI0mG7kh4GY</a></p>",
      "votes": null,
      "replies": []
    }
  ],
  "raw_markdown_by_id": {
    "622036": "**Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes**\n\nAfter perturbing, staining, and imaging cells, the open-source software CellProfiler is used to extract 1,000+ morphological features of each cell. \n\nThe collection of features is known as a profile: it reflects the phenotypic state of the cells in that sample, and can be compared to other profiles to make inferences.\n\n![](https://www.googleapis.com/download/storage/v1/b/kaggle-user-content/o/inbox%2F1107630%2F57e4fce90858ca100e71d8b806b68ce9%2Fmorphological_profiling.png?generation=1568015048361350&amp;alt=media)\n\nreference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5223290/",
    "622165": "**Introduction to Fluorescence Microscopy**\n\nFluorescence is a process in which matter absorbs light and re-emits at a different wavelength.  Fluorescence is widely used in biological microscopy.\n\n1: https://www.youtube.com/watch?v=AhzhOzgYoqw&amp;t=906s\n\n2: https://www.youtube.com/watch?v=iYoAiqufB7w\n\n3: https://www.youtube.com/watch?v=gSGq8gOLXwY\n\n4: https://www.youtube.com/watch?v=yI0mG7kh4GY"
  },
  "source": "meta"
}