{
  "id": 105046,
  "title": "The meaning of the channels",
  "url": "/competitions/recursion-cellular-image-classification/discussion/105046",
  "author_name": "Nanashi",
  "post_date": "2019-08-20T19:37:12.506000",
  "votes": 8,
  "comment_count": 4,
  "views": 0,
  "content": "<p>The 6-channel fluorescent microscopy images that comprise the RxRx1 dataset illuminate different organelles of the cell:</p>\n\n<p><code>\n1. the nucleus\n2. endoplasmic reticulum\n3. actin cytoskeleton\n4. nucleolus\n5. mitochondria\n6. golgi apparatus.\n</code></p>\n\n<p><img src=\"https://assets.website-files.com/5cb63fe47eb5472014c3dae6/5d0420ae89e22f591a352639_figure6.png\" alt=\"\"></p>\n\n<p>eg. HUVEC cells: nuclei (blue), endoplasmic reticuli (green), actin (red), nucleoli (cyan), mitochondria (magenta), and golgi apparatus (yellow). The overlap in channel content is due in part to the lack of complete spectral separation between fluorescent stains.</p>\n\n<p>source: <a href=\"https://www.rxrx.ai/\">https://www.rxrx.ai/</a></p>",
  "messages": [
    {
      "id": 603900,
      "postDate": "2019-08-20T19:37:12.507Z",
      "content": "<p>The 6-channel fluorescent microscopy images that comprise the RxRx1 dataset illuminate different organelles of the cell:</p>\n\n<p><code>\n1. the nucleus\n2. endoplasmic reticulum\n3. actin cytoskeleton\n4. nucleolus\n5. mitochondria\n6. golgi apparatus.\n</code></p>\n\n<p><img src=\"https://assets.website-files.com/5cb63fe47eb5472014c3dae6/5d0420ae89e22f591a352639_figure6.png\" alt=\"\"></p>\n\n<p>eg. HUVEC cells: nuclei (blue), endoplasmic reticuli (green), actin (red), nucleoli (cyan), mitochondria (magenta), and golgi apparatus (yellow). The overlap in channel content is due in part to the lack of complete spectral separation between fluorescent stains.</p>\n\n<p>source: <a href=\"https://www.rxrx.ai/\">https://www.rxrx.ai/</a></p>",
      "rawMarkdown": "The 6-channel fluorescent microscopy images that comprise the RxRx1 dataset illuminate different organelles of the cell:\n\n```\n1. the nucleus\n2. endoplasmic reticulum\n3. actin cytoskeleton\n4. nucleolus\n5. mitochondria\n6. golgi apparatus.\n```\n\n![](https://assets.website-files.com/5cb63fe47eb5472014c3dae6/5d0420ae89e22f591a352639_figure6.png)\n\neg. HUVEC cells: nuclei (blue), endoplasmic reticuli (green), actin (red), nucleoli (cyan), mitochondria (magenta), and golgi apparatus (yellow). The overlap in channel content is due in part to the lack of complete spectral separation between fluorescent stains.\n\nsource: https://www.rxrx.ai/",
      "votes": 8
    },
    {
      "id": 603972,
      "postDate": "2019-08-20T22:27:00.770Z",
      "content": "<p>Thanks for sharing. Probably a naive question: why are the images in the training folder in grayscale only? Does the colour matter for our model? Thanks!</p>",
      "rawMarkdown": "Thanks for sharing. Probably a naive question: why are the images in the training folder in grayscale only? Does the colour matter for our model? Thanks!",
      "votes": 1,
      "replies": [
        {
          "id": 604033,
          "postDate": "2019-08-21T00:51:26.400Z",
          "content": "<p>The color does not matter since there are not really colors. The channels are single-wavelength (ideally) filters. The channels are typically called after colors, but what those colors are don't really mean anything. You can make the channels line up by frequency, so that the shorter wavelengths are blue/violet and the longer wavelengths move towards red, but that is a choice for visualization.</p>",
          "rawMarkdown": "The color does not matter since there are not really colors. The channels are single-wavelength (ideally) filters. The channels are typically called after colors, but what those colors are don't really mean anything. You can make the channels line up by frequency, so that the shorter wavelengths are blue/violet and the longer wavelengths move towards red, but that is a choice for visualization.",
          "votes": 6
        },
        {
          "id": 604068,
          "postDate": "2019-08-21T02:10:53.133Z",
          "content": "<p>interesting. thanks!</p>",
          "rawMarkdown": "interesting. thanks!"
        }
      ]
    },
    {
      "id": 604287,
      "postDate": "2019-08-21T08:16:44.747Z",
      "rawMarkdown": "",
      "isDeleted": true
    }
  ],
  "comments": [
    {
      "id": 603972,
      "author_name": "wjsheng",
      "author_url": "",
      "post_date": "2019-08-20T22:27:00.770000",
      "content": "<p>Thanks for sharing. Probably a naive question: why are the images in the training folder in grayscale only? Does the colour matter for our model? Thanks!</p>",
      "votes": 1,
      "replies": [
        {
          "id": 604033,
          "author_name": "interneuron",
          "author_url": "",
          "post_date": "2019-08-21T00:51:26.400000",
          "content": "<p>The color does not matter since there are not really colors. The channels are single-wavelength (ideally) filters. The channels are typically called after colors, but what those colors are don't really mean anything. You can make the channels line up by frequency, so that the shorter wavelengths are blue/violet and the longer wavelengths move towards red, but that is a choice for visualization.</p>",
          "votes": 6,
          "replies": []
        },
        {
          "id": 604068,
          "author_name": "wjsheng",
          "author_url": "",
          "post_date": "2019-08-21T02:10:53.133000",
          "content": "<p>interesting. thanks!</p>",
          "votes": 0,
          "replies": []
        }
      ]
    },
    {
      "id": 604287,
      "author_name": "",
      "author_url": "",
      "post_date": "2019-08-21T08:16:44.747000",
      "content": "",
      "votes": 0,
      "replies": []
    }
  ],
  "raw_markdown_by_id": {
    "603900": "The 6-channel fluorescent microscopy images that comprise the RxRx1 dataset illuminate different organelles of the cell:\n\n```\n1. the nucleus\n2. endoplasmic reticulum\n3. actin cytoskeleton\n4. nucleolus\n5. mitochondria\n6. golgi apparatus.\n```\n\n![](https://assets.website-files.com/5cb63fe47eb5472014c3dae6/5d0420ae89e22f591a352639_figure6.png)\n\neg. HUVEC cells: nuclei (blue), endoplasmic reticuli (green), actin (red), nucleoli (cyan), mitochondria (magenta), and golgi apparatus (yellow). The overlap in channel content is due in part to the lack of complete spectral separation between fluorescent stains.\n\nsource: https://www.rxrx.ai/",
    "603972": "Thanks for sharing. Probably a naive question: why are the images in the training folder in grayscale only? Does the colour matter for our model? Thanks!",
    "604287": ""
  }
}