{
  "id": 216177,
  "title": "Why Are The Blue Channel Images SO Much Smaller?",
  "url": "/competitions/hpa-single-cell-image-classification/discussion/216177",
  "author_name": "Darien Schettler",
  "post_date": "2021-02-01T23:41:36.922000",
  "votes": 2,
  "comment_count": 4,
  "views": 0,
  "content": "<p>Hi there.</p>\n<p>As the title states, I noticed that the blue channel (nuclei) images are about a third of the size of the other channel images (Endoplasmic Reticulum, Protein of Interest, and Microtubules)</p>\n<blockquote>\n  <p><strong>---&gt; 250-450 KB for BLUE</strong><br>\n  <strong>---&gt; 1-1.5    MB for OTHER (green/yellow/red)</strong></p>\n</blockquote>\n<p>Is this a quality thing, or perhaps a nuance of the confocal microscopy? Or something else?</p>",
  "messages": [
    {
      "id": 1181488,
      "postDate": "2021-02-01T23:41:36.923Z",
      "content": "<p>Hi there.</p>\n<p>As the title states, I noticed that the blue channel (nuclei) images are about a third of the size of the other channel images (Endoplasmic Reticulum, Protein of Interest, and Microtubules)</p>\n<blockquote>\n  <p><strong>---&gt; 250-450 KB for BLUE</strong><br>\n  <strong>---&gt; 1-1.5    MB for OTHER (green/yellow/red)</strong></p>\n</blockquote>\n<p>Is this a quality thing, or perhaps a nuance of the confocal microscopy? Or something else?</p>",
      "rawMarkdown": "Hi there.\n\nAs the title states, I noticed that the blue channel (nuclei) images are about a third of the size of the other channel images (Endoplasmic Reticulum, Protein of Interest, and Microtubules)\n>**---> 250-450 KB for BLUE**\n>**---> 1-1.5    MB for OTHER (green/yellow/red)**\n\nIs this a quality thing, or perhaps a nuance of the confocal microscopy? Or something else?",
      "votes": 1
    },
    {
      "id": 1181937,
      "postDate": "2021-02-02T08:01:17.673Z",
      "content": "<p>I think the size differences are because compression is easier to do for the blue channel. The nuclei tend to be rather uniform in color intensity and do not contain any intricate patterns, so that makes it easier to encode efficiently. The nuclei are also, like <a href=\"https://www.kaggle.com/weka511\" target=\"_blank\">@weka511</a> wrote, smaller in size than the endoplasmic reticulum (yellow) and microtubules (red) so there is less information that needs to be encoded per cell.</p>\n<p>All 4 images are taken using the same microscope, using the same technique, so there shouldn't be too much of a quality difference between channels.</p>",
      "rawMarkdown": "I think the size differences are because compression is easier to do for the blue channel. The nuclei tend to be rather uniform in color intensity and do not contain any intricate patterns, so that makes it easier to encode efficiently. The nuclei are also, like @weka511 wrote, smaller in size than the endoplasmic reticulum (yellow) and microtubules (red) so there is less information that needs to be encoded per cell.\n\nAll 4 images are taken using the same microscope, using the same technique, so there shouldn't be too much of a quality difference between channels.",
      "votes": 2
    },
    {
      "id": 1181649,
      "postDate": "2021-02-02T03:22:30.583Z",
      "rawMarkdown": "",
      "isDeleted": true,
      "replies": [
        {
          "id": 1181653,
          "postDate": "2021-02-02T03:26:31.967Z",
          "rawMarkdown": "",
          "isDeleted": true
        },
        {
          "id": 1182101,
          "postDate": "2021-02-02T09:52:45.907Z",
          "content": "<p>I really like the image of the cell as a little city. Thank you for that 😄</p>\n<p>You are fully correct in that the nucleus is smaller and that ER and MT does structural work for the cell.</p>",
          "rawMarkdown": "I really like the image of the cell as a little city. Thank you for that 😄\n\nYou are fully correct in that the nucleus is smaller and that ER and MT does structural work for the cell."
        }
      ]
    }
  ],
  "comments": [
    {
      "id": 1181937,
      "author_name": "Casper Winsnes",
      "author_url": "",
      "post_date": "2021-02-02T08:01:17.673000",
      "content": "<p>I think the size differences are because compression is easier to do for the blue channel. The nuclei tend to be rather uniform in color intensity and do not contain any intricate patterns, so that makes it easier to encode efficiently. The nuclei are also, like <a href=\"https://www.kaggle.com/weka511\" target=\"_blank\">@weka511</a> wrote, smaller in size than the endoplasmic reticulum (yellow) and microtubules (red) so there is less information that needs to be encoded per cell.</p>\n<p>All 4 images are taken using the same microscope, using the same technique, so there shouldn't be too much of a quality difference between channels.</p>",
      "votes": 2,
      "replies": []
    },
    {
      "id": 1181649,
      "author_name": "",
      "author_url": "",
      "post_date": "2021-02-02T03:22:30.583000",
      "content": "",
      "votes": 0,
      "replies": [
        {
          "id": 1181653,
          "author_name": "",
          "author_url": "",
          "post_date": "2021-02-02T03:26:31.967000",
          "content": "",
          "votes": 0,
          "replies": []
        },
        {
          "id": 1182101,
          "author_name": "Casper Winsnes",
          "author_url": "",
          "post_date": "2021-02-02T09:52:45.907000",
          "content": "<p>I really like the image of the cell as a little city. Thank you for that 😄</p>\n<p>You are fully correct in that the nucleus is smaller and that ER and MT does structural work for the cell.</p>",
          "votes": 0,
          "replies": []
        }
      ]
    }
  ],
  "raw_markdown_by_id": {
    "1181488": "Hi there.\n\nAs the title states, I noticed that the blue channel (nuclei) images are about a third of the size of the other channel images (Endoplasmic Reticulum, Protein of Interest, and Microtubules)\n>**---> 250-450 KB for BLUE**\n>**---> 1-1.5    MB for OTHER (green/yellow/red)**\n\nIs this a quality thing, or perhaps a nuance of the confocal microscopy? Or something else?",
    "1181937": "I think the size differences are because compression is easier to do for the blue channel. The nuclei tend to be rather uniform in color intensity and do not contain any intricate patterns, so that makes it easier to encode efficiently. The nuclei are also, like @weka511 wrote, smaller in size than the endoplasmic reticulum (yellow) and microtubules (red) so there is less information that needs to be encoded per cell.\n\nAll 4 images are taken using the same microscope, using the same technique, so there shouldn't be too much of a quality difference between channels.",
    "1181649": ""
  }
}