{
  "id": 567045,
  "title": "Nice topics/starters for BYU LBFM2025 Contest",
  "url": "/competitions/byu-locating-bacterial-flagellar-motors-2025/discussion/567045",
  "author_name": "",
  "post_date": "2025-03-08T04:29:06.894467100Z",
  "votes": 13,
  "comment_count": 2,
  "views": 0,
  "content": "<p>A good list of resources for the competition:</p>\n<ul>\n<li><strong>Bioimage Analysis Basics</strong>: <a href=\"https://opensourceimaging.org/project/python-for-microscopy/\" target=\"_blank\">Python for Microscopy (OpenSource Imaging)</a>  </li>\n<li><strong>Flagellar Motor Biology</strong>: Review paper <a href=\"https://www.nature.com/articles/sj.jid.5700897\" target=\"_blank\">\"Self-assembly and type III protein export of the bacterial flagellum\"</a> by Minamino &amp; Namba  </li>\n<li><strong>Segmentation Models</strong>: Kaggle Notebook <a href=\"https://www.kaggle.com/code/ateplyuk/u-net-with-keras\" target=\"_blank\">\"U-Net with TensorFlow\"</a>  </li>\n<li><strong>Preprocessing Microscopy Data</strong>: <a href=\"https://cellprofiler.org/tutorials\" target=\"_blank\">CellProfiler Tutorial</a>  </li>\n<li><strong>Advanced Techniques</strong>: Paper <a href=\"https://www.nature.com/articles/s41592-019-0403-1\" target=\"_blank\">\"Deep Learning for Cellular Image Analysis\"</a> in <em>Nature Methods</em>  </li>\n</ul>\n<h3><strong>Core Biological Context</strong></h3>\n<ul>\n<li><strong>Torque Generation &amp; Motor Dynamics</strong>: <a href=\"https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.911114/full\" target=\"_blank\">\"The Bacterial Flagellar Motor: Insights Into Torque Generation\"</a> (Frontiers, 2022) – Explores cryo-ET insights and stator-rotor interactions.  </li>\n<li><strong>Ion Motive Force Mechanics</strong>: <a href=\"https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.659464/full\" target=\"_blank\">\"The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor\"</a> (Frontiers, 2021) – Links IMF to motor speed and torque.  </li>\n</ul>\n<h3><strong>Image Analysis Foundations</strong></h3>\n<ul>\n<li><strong>Beginner’s Guide</strong>: <a href=\"https://www.bioimagingguide.org/03_Image_analysis/Resources.html\" target=\"_blank\">Peter Bankhead’s Intro to Bioimage Analysis</a> – Python/ImageJ exercises for microscopy.  </li>\n<li><strong>Workflow Design</strong>: <a href=\"https://www.ebi.ac.uk/training/events/methods-bioimage-analysis\" target=\"_blank\">EMBL-EBI Bioimage Analysis Webinar</a> – Covers object detection, quality control, and tools.  </li>\n<li><strong>Tool Repository</strong>: <a href=\"https://www.bioimagingguide.org/03_Image_analysis/Resources.html\" target=\"_blank\">BioImage Informatics Index</a> – Searchable database for analysis workflows.  </li>\n</ul>\n<h3><strong>Machine Learning &amp; Deep Learning</strong></h3>\n<ul>\n<li><strong>Zero-Cost DL Tools</strong>: <a href=\"https://www.nature.com/articles/s41467-021-22518-0\" target=\"_blank\">ZeroCostDL4Mic</a> (Nature, 2021) – Pre-trained models for microscopy.  </li>\n<li><strong>ML Applications</strong>: <a href=\"https://www.leica-microsystems.com/science-lab/life-science/using-machine-learning-in-microscopy-image-analysis/\" target=\"_blank\">Leica Guide to ML in Microscopy</a> – Pixel classification, segmentation best practices.  </li>\n<li><strong>3D Analysis</strong>: <a href=\"https://www.frontiersin.org/journals/computer-science/articles/10.3389/fcomp.2022.931939/full\" target=\"_blank\">Hybrid Deep Learning/Bayesian Tracking</a> (Frontiers, 2022) – For 3D object reconstruction.  </li>\n</ul>",
  "messages": [
    {
      "id": "3144189",
      "postDate": "03/08/2025 04:29:06",
      "content": "<p>A good list of resources for the competition:</p>\n<ul>\n<li><strong>Bioimage Analysis Basics</strong>: <a href=\"https://opensourceimaging.org/project/python-for-microscopy/\" target=\"_blank\">Python for Microscopy (OpenSource Imaging)</a>  </li>\n<li><strong>Flagellar Motor Biology</strong>: Review paper <a href=\"https://www.nature.com/articles/sj.jid.5700897\" target=\"_blank\">\"Self-assembly and type III protein export of the bacterial flagellum\"</a> by Minamino &amp; Namba  </li>\n<li><strong>Segmentation Models</strong>: Kaggle Notebook <a href=\"https://www.kaggle.com/code/ateplyuk/u-net-with-keras\" target=\"_blank\">\"U-Net with TensorFlow\"</a>  </li>\n<li><strong>Preprocessing Microscopy Data</strong>: <a href=\"https://cellprofiler.org/tutorials\" target=\"_blank\">CellProfiler Tutorial</a>  </li>\n<li><strong>Advanced Techniques</strong>: Paper <a href=\"https://www.nature.com/articles/s41592-019-0403-1\" target=\"_blank\">\"Deep Learning for Cellular Image Analysis\"</a> in <em>Nature Methods</em>  </li>\n</ul>\n<h3><strong>Core Biological Context</strong></h3>\n<ul>\n<li><strong>Torque Generation &amp; Motor Dynamics</strong>: <a href=\"https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.911114/full\" target=\"_blank\">\"The Bacterial Flagellar Motor: Insights Into Torque Generation\"</a> (Frontiers, 2022) – Explores cryo-ET insights and stator-rotor interactions.  </li>\n<li><strong>Ion Motive Force Mechanics</strong>: <a href=\"https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.659464/full\" target=\"_blank\">\"The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor\"</a> (Frontiers, 2021) – Links IMF to motor speed and torque.  </li>\n</ul>\n<h3><strong>Image Analysis Foundations</strong></h3>\n<ul>\n<li><strong>Beginner’s Guide</strong>: <a href=\"https://www.bioimagingguide.org/03_Image_analysis/Resources.html\" target=\"_blank\">Peter Bankhead’s Intro to Bioimage Analysis</a> – Python/ImageJ exercises for microscopy.  </li>\n<li><strong>Workflow Design</strong>: <a href=\"https://www.ebi.ac.uk/training/events/methods-bioimage-analysis\" target=\"_blank\">EMBL-EBI Bioimage Analysis Webinar</a> – Covers object detection, quality control, and tools.  </li>\n<li><strong>Tool Repository</strong>: <a href=\"https://www.bioimagingguide.org/03_Image_analysis/Resources.html\" target=\"_blank\">BioImage Informatics Index</a> – Searchable database for analysis workflows.  </li>\n</ul>\n<h3><strong>Machine Learning &amp; Deep Learning</strong></h3>\n<ul>\n<li><strong>Zero-Cost DL Tools</strong>: <a href=\"https://www.nature.com/articles/s41467-021-22518-0\" target=\"_blank\">ZeroCostDL4Mic</a> (Nature, 2021) – Pre-trained models for microscopy.  </li>\n<li><strong>ML Applications</strong>: <a href=\"https://www.leica-microsystems.com/science-lab/life-science/using-machine-learning-in-microscopy-image-analysis/\" target=\"_blank\">Leica Guide to ML in Microscopy</a> – Pixel classification, segmentation best practices.  </li>\n<li><strong>3D Analysis</strong>: <a href=\"https://www.frontiersin.org/journals/computer-science/articles/10.3389/fcomp.2022.931939/full\" target=\"_blank\">Hybrid Deep Learning/Bayesian Tracking</a> (Frontiers, 2022) – For 3D object reconstruction.  </li>\n</ul>",
      "rawMarkdown": "A good list of resources for the competition:\n- **Bioimage Analysis Basics**: [Python for Microscopy (OpenSource Imaging)](https://opensourceimaging.org/project/python-for-microscopy/)  \n- **Flagellar Motor Biology**: Review paper [\"Self-assembly and type III protein export of the bacterial flagellum\"](https://www.nature.com/articles/sj.jid.5700897) by Minamino & Namba  \n- **Segmentation Models**: Kaggle Notebook [\"U-Net with TensorFlow\"](https://www.kaggle.com/code/ateplyuk/u-net-with-keras)  \n- **Preprocessing Microscopy Data**: [CellProfiler Tutorial](https://cellprofiler.org/tutorials)  \n- **Advanced Techniques**: Paper [\"Deep Learning for Cellular Image Analysis\"](https://www.nature.com/articles/s41592-019-0403-1) in *Nature Methods*  \n\n### **Core Biological Context**  \n- **Torque Generation & Motor Dynamics**: [\"The Bacterial Flagellar Motor: Insights Into Torque Generation\"](https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.911114/full) (Frontiers, 2022) – Explores cryo-ET insights and stator-rotor interactions.  \n- **Ion Motive Force Mechanics**: [\"The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor\"](https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.659464/full) (Frontiers, 2021) – Links IMF to motor speed and torque.  \n\n### **Image Analysis Foundations**  \n- **Beginner’s Guide**: [Peter Bankhead’s Intro to Bioimage Analysis](https://www.bioimagingguide.org/03_Image_analysis/Resources.html) – Python/ImageJ exercises for microscopy.  \n- **Workflow Design**: [EMBL-EBI Bioimage Analysis Webinar](https://www.ebi.ac.uk/training/events/methods-bioimage-analysis) – Covers object detection, quality control, and tools.  \n- **Tool Repository**: [BioImage Informatics Index](https://www.bioimagingguide.org/03_Image_analysis/Resources.html) – Searchable database for analysis workflows.  \n\n### **Machine Learning & Deep Learning**  \n- **Zero-Cost DL Tools**: [ZeroCostDL4Mic](https://www.nature.com/articles/s41467-021-22518-0) (Nature, 2021) – Pre-trained models for microscopy.  \n- **ML Applications**: [Leica Guide to ML in Microscopy](https://www.leica-microsystems.com/science-lab/life-science/using-machine-learning-in-microscopy-image-analysis/) – Pixel classification, segmentation best practices.  \n- **3D Analysis**: [Hybrid Deep Learning/Bayesian Tracking](https://www.frontiersin.org/journals/computer-science/articles/10.3389/fcomp.2022.931939/full) (Frontiers, 2022) – For 3D object reconstruction.",
      "votes": null
    },
    {
      "id": "3154226",
      "postDate": "03/19/2025 17:06:09",
      "content": "<p>Thanks for your comments and votes!</p>",
      "rawMarkdown": "Thanks for your comments and votes!",
      "votes": null
    },
    {
      "id": "3158003",
      "postDate": "03/24/2025 06:16:49",
      "content": "<p>What additional inputs can you share for this list?</p>",
      "rawMarkdown": "What additional inputs can you share for this list?",
      "votes": null
    }
  ],
  "comments": [
    {
      "id": 3154226,
      "author_name": "kalilurrahman",
      "author_url": "",
      "post_date": "03/19/2025 17:06:09",
      "content": "<p>Thanks for your comments and votes!</p>",
      "votes": null,
      "replies": []
    },
    {
      "id": 3158003,
      "author_name": "kalilurrahman",
      "author_url": "",
      "post_date": "03/24/2025 06:16:49",
      "content": "<p>What additional inputs can you share for this list?</p>",
      "votes": null,
      "replies": []
    }
  ],
  "raw_markdown_by_id": {
    "3144189": "A good list of resources for the competition:\n- **Bioimage Analysis Basics**: [Python for Microscopy (OpenSource Imaging)](https://opensourceimaging.org/project/python-for-microscopy/)  \n- **Flagellar Motor Biology**: Review paper [\"Self-assembly and type III protein export of the bacterial flagellum\"](https://www.nature.com/articles/sj.jid.5700897) by Minamino & Namba  \n- **Segmentation Models**: Kaggle Notebook [\"U-Net with TensorFlow\"](https://www.kaggle.com/code/ateplyuk/u-net-with-keras)  \n- **Preprocessing Microscopy Data**: [CellProfiler Tutorial](https://cellprofiler.org/tutorials)  \n- **Advanced Techniques**: Paper [\"Deep Learning for Cellular Image Analysis\"](https://www.nature.com/articles/s41592-019-0403-1) in *Nature Methods*  \n\n### **Core Biological Context**  \n- **Torque Generation & Motor Dynamics**: [\"The Bacterial Flagellar Motor: Insights Into Torque Generation\"](https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.911114/full) (Frontiers, 2022) – Explores cryo-ET insights and stator-rotor interactions.  \n- **Ion Motive Force Mechanics**: [\"The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor\"](https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2021.659464/full) (Frontiers, 2021) – Links IMF to motor speed and torque.  \n\n### **Image Analysis Foundations**  \n- **Beginner’s Guide**: [Peter Bankhead’s Intro to Bioimage Analysis](https://www.bioimagingguide.org/03_Image_analysis/Resources.html) – Python/ImageJ exercises for microscopy.  \n- **Workflow Design**: [EMBL-EBI Bioimage Analysis Webinar](https://www.ebi.ac.uk/training/events/methods-bioimage-analysis) – Covers object detection, quality control, and tools.  \n- **Tool Repository**: [BioImage Informatics Index](https://www.bioimagingguide.org/03_Image_analysis/Resources.html) – Searchable database for analysis workflows.  \n\n### **Machine Learning & Deep Learning**  \n- **Zero-Cost DL Tools**: [ZeroCostDL4Mic](https://www.nature.com/articles/s41467-021-22518-0) (Nature, 2021) – Pre-trained models for microscopy.  \n- **ML Applications**: [Leica Guide to ML in Microscopy](https://www.leica-microsystems.com/science-lab/life-science/using-machine-learning-in-microscopy-image-analysis/) – Pixel classification, segmentation best practices.  \n- **3D Analysis**: [Hybrid Deep Learning/Bayesian Tracking](https://www.frontiersin.org/journals/computer-science/articles/10.3389/fcomp.2022.931939/full) (Frontiers, 2022) – For 3D object reconstruction.",
    "3154226": "Thanks for your comments and votes!",
    "3158003": "What additional inputs can you share for this list?"
  },
  "source": "meta"
}